The textiles industry is a substantial contributor to environmental impacts through the production, processing, use, and end-of-life of garments. Wool is a high value, natural, and renewable fibre that is used to produce a wide range of garments, from active leisure wear to formal wear, and represents a small segment of the global fashion industry. Woollen garments are produced by long, global value chains extending from the production of ‘greasy’ wool on sheep farms, through processing to garment make-up, retail, consumer use, and end-of-life. To date, there have been limited life cycle assessment (LCA) studies on the environmental impacts of the full supply chain or use phase of garments, with the majority of wool LCA studies focusing on a segment of the supply chain. This study aimed to address this knowledge gap via a cradle-to-grave LCA of a woollen garment.